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Plasticity of Cyanobacterial Thylakoid Microdomains Under Variable Light Conditions
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SYSNO ASEP 0535989 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plasticity of Cyanobacterial Thylakoid Microdomains Under Variable Light Conditions Author(s) Canonico, Myriam (MBU-M) ORCID
Konert, Grzegorz (MBU-M) ORCID
Kaňa, Radek (MBU-M) RID, ORCIDArticle number 586543 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 11, NOV 12 (2020)Number of pages 11 s. Language eng - English Country CH - Switzerland Keywords photosynthesis ; thylakoid membrane ; microdomains and rafts ; membrane organization Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects GA19-11494S GA ČR - Czech Science Foundation (CSF) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000592204300001 EID SCOPUS 85096703101 DOI 10.3389/fpls.2020.586543 Annotation Photosynthetic light reactions proceed in thylakoid membranes (TMs) due to the activity of pigment-protein complexes. These complexes are heterogeneously organized into granal/stromal thylakoids (in plants) or into recently identified cyanobacterial microdomains (MDs). MDs are characterized by specific ratios of photosystem I (PSI), photosystem II (PSII), and phycobilisomes (PBS) and they are visible as sub-micrometer sized areas with different fluorescence ratios. In this report, the process of long-term plasticity in cyanobacterial thylakoid MDs has been explored under variable growth light conditions using Synechocystis sp. PCC6803 expressing YFP tagged PSI. TM organization into MDs has been observed for all categorized shapes of cells independently of their stage in cell cycle. The heterogeneous PSI, PSII, and PBS thylakoid areas were also identified under two types of growth conditions: at continuous light (CL) and at light-dark (L-D) cycle. The acclimation from CL to L-D cycle changed spatial distribution of photosystems, in particular PSI became more evenly distributed in thylakoids under L-D cycle. The process of the spatial PSI (and partially also PSII) redistribution required 1 week and was accompanied by temporal appearance of PBS decoupling probably caused by the re-organization of photosystems. The overall acclimation we observed was defined as TM plasticity as it resembles higher plants grana/stroma reorganization at variable growth light conditions. In addition, we observed large cell to cell variability in the actual MDs organization. It leads us to suggest that the plasticity, and cell to cell variability in MDs could be a manifestation of phenotypic heterogeneity, a recently broadly discussed phenomenon for prokaryotes. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.frontiersin.org/articles/10.3389/fpls.2020.586543/full
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