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The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies
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SYSNO ASEP 0533112 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in yeast colonies Author(s) Maršíková, J. (CZ)
Pavlíčková, Martina (MBU-M) ORCID
Wilkinson, D. (CZ)
Váchová, Libuše (MBU-M) RID, ORCID
Hlaváček, Otakar (MBU-M) RID, ORCID
Hatáková, L. (CZ)
Palková, Z. (CZ)Source Title Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
Roč. 117, č. 26 (2020), s. 15123-15131Number of pages 9 s. Language eng - English Country US - United States Keywords yeast multicellularity ; interference competition ; interaction-specific fitness inequality Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LTAUSA18162 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000565729700013 EID SCOPUS 85087466656 DOI 10.1073/pnas.1922076117 Annotation Yeast form complex highly organized colonies in which cells undergo spatiotemporal phenotypic differentiation in response to local gradients of nutrients, metabolites, and specific signaling molecules. Colony fitness depends on cell interactions, cooperation, and the division of labor between differentiated cell subpopulations. Here, we describe the regulation and dynamics of the expansion of papillae that arise during colony aging, which consist of cells that overcome colony regulatory rules and disrupt the synchronized colony structure. We show that papillae specifically expand within the U cell sub-population in differentiated colonies. Papillae emerge more frequently in some strains than in others. Genomic analyses further revealed that the Whi2p-Psr1p/Psr2p complex (WPPC) plays a key role in papillae expansion. We show that cells lacking a functional WPPC have a sizable interaction-specific fitness advantage attributable to production of and resistance to a diffusible compound that inhibits growth of other cells. Competitive superiority and high relative fitness of whi2 and psr1psr2 strains are particularly pronounced in dense spatially structured colonies and are independent of TORC1 and Msn2p/Msn4p regulators previously associated with the WPPC function. The WPPC function, described here, might be a regulatory mechanism that balances cell competition and cooperation in dense yeast populations and, thus, contributes to cell synchronization, pattern formation, and the expansion of cells with a competitive fitness advantage. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://www.pnas.org/content/117/26/15123
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