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DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles
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SYSNO ASEP 0532304 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title DNA Methylation Profiles in a Group of Workers Occupationally Exposed to Nanoparticles Author(s) Rössnerová, Andrea (UEM-P) RID
Hoňková, Kateřina (UEM-P)
Pelclová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Hubáček, J. A. (CZ)
Chvojková, Irena (UEM-P)
Vrbová, Kristýna (UEM-P)
Rössner ml., Pavel (UEM-P) RID, ORCID
Topinka, Jan (UEM-P) RID, ORCID
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Lischková, L. (CZ)
Klusáčková, P. (CZ)
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Ondráčková, Lucie (UCHP-M) RID, ORCID, SAI
Koštejn, Martin (UCHP-M) RID, SAI, ORCID
Kléma, J. (CZ)
Dvořáčková, Š. (CZ)Article number 2420 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 7 (2020)Number of pages 20 s. Language eng - English Country CH - Switzerland Keywords 850K microarray ; CpG sites ; DNA methylation Subject RIV DN - Health Impact of the Environment Quality OECD category Occupational health Subject RIV - cooperation Institute of Chemical Process Fundamentals - Health Impact of the Environment Quality R&D Projects GA18-02079S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000535574200162 EID SCOPUS 85083042156 DOI 10.3390/ijms21072420 Annotation The risk of exposure to nanoparticles (NPs) has rapidly increased during the last decade due to the vast use of nanomaterials (NMs) in many areas of human life. Despite this fact, human biomonitoring studies focused on the effect of NP exposure on DNA alterations are still rare. Furthermore, there are virtually no epigenetic data available. In this study, we investigated global and gene-specific DNA methylation profiles in a group of 20 long-term (mean 14.5 years) exposed, nanocomposite, research workers and in 20 controls. Both groups were sampled twice/day (pre-shift and post-shift) in September 2018. We applied Infinium Methylation Assay, using the Infinium MethylationEPIC BeadChips with more than 850,000 CpG loci, for identification of the DNA methylation pattern in the studied groups. Aerosol exposure monitoring, including two nanosized fractions, was also performed as proof of acute NP exposure. The obtained array data showed significant differences in methylation between the exposed and control groups related to long-term exposure, specifically 341 CpG loci were hypomethylated and 364 hypermethylated. The most significant CpG differences were mainly detected in genes involved in lipid metabolism, the immune system, lung functions, signaling pathways, cancer development and xenobiotic detoxification. In contrast, short-term acute NP exposure was not accompanied by DNA methylation changes. In summary, long-term (years) exposure to NP is associated with DNA epigenetic alterations. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2021 Electronic address https://www.mdpi.com/1422-0067/21/7/2420
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