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Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase
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SYSNO ASEP 0531340 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Modulation of non-bilayer lipid phases and the structure and functions of thylakoid membranes: effects on the water-soluble enzyme violaxanthin de-epoxidase Author(s) Dlouhý, O. (CZ)
Kurasová, Irena (UEK-B)
Karlický, Václav (UEK-B) ORCID, RID, SAI
Javorník, U. (CZ)
Šket, P. (SI)
Petrova, N. Z. (BG)
Krumova, S. B. (BG)
Plavec, J. (SI)
Ughy, B. (HU)
Špunda, Vladimír (UEK-B) ORCID, SAI, RID
Garab, G. (HU)Number of authors 11 Article number 11959 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords non-bilayer lipids ; 31p-nmr spectroscopy ; thylakoid membranes ; violaxanthin de-epoxidase Subject RIV ED - Physiology OECD category Plant sciences, botany R&D Projects EF16_019/0000797 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Method of publishing Open access Institutional support UEK-B - RVO:86652079 UT WOS 000553550700029 EID SCOPUS 85088278026 DOI 10.1038/s41598-020-68854-x Annotation The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem of membrane biology. Non-bilayer lipids are present in large amounts in all membranes, in energy-converting membranes they constitute about half of their total lipid content—yet their functional state is a bilayer. In vitro experiments revealed that the functioning of the water-soluble violaxanthin de-epoxidase (VDE) enzyme of plant thylakoids requires the presence of a non-bilayer lipid phase. 31P-NMR spectroscopy has provided evidence on lipid polymorphism in functional thylakoid membranes. Here we reveal reversible pH- and temperature-dependent changes of the lipid-phase behaviour, particularly the flexibility of isotropic non-lamellar phases, of isolated spinach thylakoids. These reorganizations are accompanied by changes in the permeability and thermodynamic parameters of the membranes and appear to control the activity of VDE and the photoprotective mechanism of non-photochemical quenching of chlorophyll-a fluorescence. The data demonstrate, for the first time in native membranes, the modulation of the activity of a water-soluble enzyme by a non-bilayer lipid phase. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2021 Electronic address https://www.nature.com/articles/s41598-020-68854-x
Number of the records: 1