Number of the records: 1
A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor)
- 1.0531246 - MBÚ 2021 RIV US eng J - Journal Article
Pospíšil, Jiří - Strunin, Dmytro - Ziková, Alice - Hubálek, Martin - Vohradský, Jiří
A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor).
Proteomics. Roč. 20, č. 14 (2020), č. článku 2000032. ISSN 1615-9853. E-ISSN 1615-9861
R&D Projects: GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LM2015047; GA MŠMT(CZ) LM2018131
Research Infrastructure: C4SYS - 90055; ELIXIR-CZ - 90047; ELIXIR-CZ II - 90131
Institutional support: RVO:61388971 ; RVO:61388963
Keywords : computational modeling * mass spectrometry * mRNA
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology; Analytical chemistry (UOCHB-X)
Impact factor: 3.984, year: 2020
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.202000032
In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteriaStreptomyces coelicolor(S. coelicolorM145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results reveal that about one third of protein/mRNA synthesis profiles are well correlated while another third are correlated negatively. Functional analysis of the highly correlated groups is presented. Based on numerical simulation, the negative correlation between protein and mRNA is shown to be caused by the difference between the rate of translation and protein degradation.
Permanent Link: http://hdl.handle.net/11104/0310042
Number of the records: 1