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Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p '-DDT and p,p '-DDE
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SYSNO ASEP 0524267 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p '-DDT and p,p '-DDE Author(s) Pavlíková, N. (CZ)
Daniel, P. (CZ)
Šrámek, J. (CZ)
Jelínek, M. (CZ)
Šrámková, V. (CZ)
Němcová, V. (CZ)
Balušíková, K. (CZ)
Halada, Petr (MBU-M) RID, ORCID
Kovář, J. (CZ)Article number 18026 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 9, DEC 2 (2019)Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords persistent organic pollutants ; endoplasmic-reticulum stress ; breast-cancer cells Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000500573100001 EID SCOPUS 85076019187 DOI 10.1038/s41598-019-54579-z Annotation Persistent organochlorine pollutants (POPs) gradually accumulate in the human organism due to their presence in the environment. Some studies have described a correlation between the level of POPs in the human body and the incidence of diabetes, but we know little about the direct effect of POPs on pancreatic beta-cells. We exposed pancreatic beta-cells INS1E to non-lethal concentrations of p,p'-DDT (1,1'-(2,2,2-Trichloroethane-1,1-diyl)bis(4-chlorobenzene)) and p,p'-DDE (1,1'-(2,2-dichloroethene-1,1-diyl)bis(4-chlorobenzene)) for 1 month, and assessed changes in protein expression and the intracellular insulin level. 2-D electrophoresis revealed 6 proteins with changed expression in cells exposed to p,p'-DDT or p,p'-DDE. One of the detected proteins - vitamin D-binding protein (VDBP) - was upregulated in both cells exposed to p,p'-DDT, and cells exposed to p,p'-DDE. Both exposures to pollutants reduced the intracellular level of insulin mRNA, proinsulin, and insulin monomer, p,p'-DDT also slightly reduced the level of hexameric insulin. Overexpression of VDBP caused by the stable transfection of beta-cells with the gene for VDBP decreased both the proinsulin and hexameric insulin level in beta-cells similarly to the reduction detected in cells exposed to p,p'-DDT. Our data suggest that in the cells exposed to p,p'-DDT and p,p'-DDE, the increased VDBP protein level decreased the proinsulin expression in an unknown mechanism. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2021 Electronic address https://d360prx.biomed.cas.cz:2645/articles/s41598-019-54579-z.pdf
Number of the records: 1