A novel voltammetric approach to the detection of primary bile acids in serum samples

0524179 - ÚOCHB 2021 RIV CH eng J - Journal Article
Klouda, J. - Nesměrák, K. - Kočovský, Pavel - Barek, J. - Schwarzová-Pecková, K.
A novel voltammetric approach to the detection of primary bile acids in serum samples.
Bioelectrochemistry. Roč. 134, Aug (2020), č. článku 107539. ISSN 1567-5394. E-ISSN 1878-562X
Institutional support: RVO:61388963
Keywords : boron-doped diamond electrode * dehydration * human serum * primary bile acids * solid phase extraction * voltammetry
OECD category: Analytical chemistry
Impact factor: 5.373, year: 2020
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S1567539419308266?via%3Dihub

An innovative voltammetric approach to the detection of cholic and chenodeoxycholic acids is presented. These two primary bile acids are important biomarkers of liver function in humans and are involved in many physiological processes in the human body. Herein we describe a way to reproducibly convert the hard-to-detect bile acid molecule into an easily detectable derivative in situ using 0.1 M HClO4 in acetonitrile (water content 0.55%). Under these conditions the bile acids are dehydrated and the resulting alkenes can be subsequently oxidized electrochemically on polished boron-doped diamond electrode under unchanged conditions at approximately +1.2 V vs. Ag/AgNO3 in acetonitrile. After optimization, differential pulse voltammetry provides competitive limits of detection of 0.5 µM and 1.0 µM for cholic and chenodeoxycholic acid, respectively, with a linear course of calibration dependency to the minimum of 80 µM. The method was applied for detection of cholic and chenodeoxycholic acids in artificial and human serum samples using single solid phase extraction on C-18 cartridge for preliminary separation of the analytes. High recoveries of 80–90% were consistently obtained by the proposed voltammetric method and reference HPLC with fluorescence detection for human serum samples, confirming good selectivity for real-life samples.
Permanent Link: http://hdl.handle.net/11104/0308548