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Steady-state levels of cytokinins and their derivatives may serve as a unique classifier of arabidopsis ecotypes
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SYSNO ASEP 0523574 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Steady-state levels of cytokinins and their derivatives may serve as a unique classifier of arabidopsis ecotypes Author(s) Samsonová, Z. (CZ)
Kiran, N.S. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Spyroglou, I. (CZ)
Skalák, J. (CZ)
Hejátko, J. (CZ)
Gloser, V. (CZ)Number of authors 7 Article number 116 Source Title Plants. - : MDPI
Roč. 9, č. 1 (2020)Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords Abscisic acid ; Cytokinin glucosides ; Cytokinin metabolism ; Cytokinins ; Indole-3-acetic acid ; Single nucleotide polymorphism Subject RIV EB - Genetics ; Molecular Biology OECD category GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming R&D Projects LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000513238700116 EID SCOPUS 85078500965 DOI 10.3390/plants9010116 Annotation We determined steady-state (basal) endogenous levels of three plant hormones (abscisic acid, cytokinins and indole-3-acetic acid) in a collection of thirty different ecotypes of Arabidopsis that represent a broad genetic variability within this species. Hormone contents were analysed separately in plant shoots and roots after 21 days of cultivation on agar plates in a climate-controlled chamber. Using advanced statistical and machine learning methods, we tested if basal hormonal levels can be considered a unique ecotype-specific classifier. We also explored possible relationships between hormone levels and the prevalent environmental conditions in the site of origin for each ecotype. We found significant variations in basal hormonal levels and their ratios in both root and shoot among the ecotypes. We showed the prominent position of cytokinins (CK) among the other hormones. We found the content of CK and CK metabolites to be a reliable ecotype-specific identifier. Correlation with the mean temperature at the site of origin and the large variation in basal hormonal levels suggest that the high variability may potentially be in response to environmental factors. This study provides a starting point for ecotype-specific genetic maps of the CK metabolic and signalling network to explore its contribution to the adaptation of plants to local environmental conditions. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2020 Electronic address http://doi.org/10.3390/plants9010116
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