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Ecotoxicology impact of silica-coated CdSe/ZnS quantum dots internalized in Chlamydomonas reinhardtii algal cells
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SYSNO ASEP 0508300 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ecotoxicology impact of silica-coated CdSe/ZnS quantum dots internalized in Chlamydomonas reinhardtii algal cells Author(s) Elzorkany, H.E. (EG)
Farghali, M.A. (EG)
Hassan, M. A. (EG)
El-sayed, Kh. (EG)
Canonico, Myriam (MBU-M) ORCID
Konert, Grzegorz (MBU-M) ORCID
Farroh, K. (EG)
Elshoky, H.A. (EG)
Kaňa, Radek (MBU-M) RID, ORCIDSource Title Science of the Total Environment. - : Elsevier - ISSN 0048-9697
Roč. 666, MAY 20 (2019), s. 480-489Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords Photosynthetic organisms ; Nanocrystals ; Confocal microscopy Subject RIV EH - Ecology, Behaviour OECD category Environmental sciences (social aspects to be 5.7) R&D Projects GA16-10088S GA ČR - Czech Science Foundation (CSF) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000463180700047 EID SCOPUS 85061824966 DOI 10.1016/j.scitotenv.2019.02.274 Annotation The use of quantum dots (QD) in various medical and industrial applications may cause these nanoparticles to leak into waterways and subsequently enter the food chain. Therefore, if we intend to use QD, we must first know their potential environmental implications. In this work, cadmium selenide/zinc sulfide core/shell QD were synthesized, and then, biocompatible, water-dispersed QD were coated with silica (Si-QD). The QD were characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM) combined with energy-dispersive X-ray spectroscopy (EDX), and UV-Vis absorption analysis, which revealed that these surface-engineered QD have a highly crystalline, homogeneous spherical shape measuring approximately 25 nm. The cytotoxicity of the nanoparticles in the green algae Chlamydomonas reinhardtii was studied by incubating the algae cells with Si-QD and determining the optical density of algal cell culture, cell counts, and cells sizes by microflow cytometry. These measurements indicated that Si-QD are biocompatible up to a concentration of 25 ng/ml. Finally, the cellular uptake of Si-QD into C. reinhardtii was monitored by confocal laser scanning microscopy (CLSM). In conclusion, our results reveal that surface-engineered Cd-QD can penetrate the cells of aquatic organisms, which ensures a serious impact on the food chain and consequently the environment. On the other hand, the results also highlight a new potential method for bioremediation of Cd-QD by green algae, especially C. reinhardtii. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2020 Electronic address https://www.sciencedirect.com/science/article/pii/S0048969719307727?via%3Dihub
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