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Activity and inactivity of moth sex chromosomes in somatic and meiotic cells
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SYSNO ASEP 0507648 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Activity and inactivity of moth sex chromosomes in somatic and meiotic cells Author(s) Traut, W. (DE)
Schubert, V. (DE)
Dalíková, Martina (BC-A) RID, ORCID
Marec, František (BC-A) RID, ORCID
Sahara, K. (JP)Number of authors 5 Source Title Chromosoma. - : Springer - ISSN 0009-5915
Roč. 128, č. 4 (2019), s. 533-545Number of pages 13 s. Language eng - English Country US - United States Keywords heterochromatin ; sex chromatin ; germline-limited activity Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects GA17-13713S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 000495294600003 EID SCOPUS 85070911671 DOI 10.1007/s00412-019-00722-8 Annotation Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2020 Electronic address https://link.springer.com/article/10.1007%2Fs00412-019-00722-8
Number of the records: 1