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Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells
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SYSNO ASEP 0501540 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells Author(s) Perez-Guaita, D. (AU)
Kochan, K. (AU)
Batty, M. (AU)
Doerig, C. (AU)
Garcia-Bustos, J. (AU)
Espinoza Herrera, Shirly J. (FZU-D) ORCID
McNaughton, D. (AU)
Heraud, P. (AU)
Wood, Bayden R. (AU)Number of authors 9 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 90, č. 5 (2018), s. 3140-3148Number of pages 9 s. Language eng - English Country US - United States Keywords IR spectroscopy ; structural lipids ; AFM-IR ; hemozoin ; proteins ; quantification ; localization ; conformation ; resolution ; parasite Subject RIV BL - Plasma and Gas Discharge Physics OECD category Fluids and plasma physics (including surface physics) R&D Projects EF15_003/0000447 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LQ1606 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FZU-D - RVO:68378271 UT WOS 000427095700026 EID SCOPUS 85043234845 DOI 10.1021/acs.analchem.7b04318 Annotation Atomic force microscopy-infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nanoscale. AFM-IR maps are acquired using a single wavenumber value: they show either the absorbance plotted against a single wavenumber value or a ratio of two absorbance values. Here, we implement multivariate image analysis to generate multivariate AFM-IR maps and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development. This was achieved by converting the discrete spectral points into a multispectral line spectrum prior to multivariate image reconstruction. The approach was used to generate compositional maps of subcellular structures in the parasites, including the food vacuole, lipid inclusions, and the nucleus, on the basis of the intensity of hemozoin, hemoglobin, lipid, and DNA IR marker bands, respectively. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2019
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