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Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics
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SYSNO ASEP 0495307 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics Author(s) Hafidh, Said (UEB-Q) RID, ORCID
Potěšil, D. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Fíla, Jan (UEB-Q) RID, ORCID
Michailidis, Christos (UEB-Q) ORCID
Herrmannová, Anna (MBU-M) RID, ORCID
Feciková, Jana (UEB-Q) ORCID
Ischebeck, T. (DE)
Valášek, Leoš Shivaya (MBU-M) RID, ORCID
Zdráhal, Z. (CZ)
Honys, David (UEB-Q) RID, ORCIDNumber of authors 11 Source Title Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 178, č. 1 (2018), s. 258-282Number of pages 25 s. Language eng - English Country US - United States Keywords differentially expressed proteins ; male gametophyte development ; messenger-rna transport ; tube growth ; nicotiana-tabacum ; gene-expression ; arabidopsis-thaliana ; mature pollen ; proteomic analysis ; processing bodies Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015043 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-06238S GA ČR - Czech Science Foundation (CSF) GA18-02448S GA ČR - Czech Science Foundation (CSF) GA17-23183S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 ; MBU-M - RVO:61388971 UT WOS 000444004100022 EID SCOPUS 85054564041 DOI 10.1104/pp.18.00648 Annotation Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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