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Thickness determination of a cathodoluminescence active nanoparticles by means of Quantitative STEM imaging
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SYSNO ASEP 0494375 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Thickness determination of a cathodoluminescence active nanoparticles by means of Quantitative STEM imaging Author(s) Skoupý, Radim (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAINumber of authors 2 Source Title Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Proceedings of the 16th International Seminar. - Brno : Institute of Scientific Instruments The Czech Academy of Sciences, 2018 - ISBN 978-80-87441-23-7 Pages s. 74-76 Number of pages 3 s. Publication form Print - P Action Recent Trends in Charged Particle Optics and Surface Physics Instrumentation Event date 04.06.2018 - 08.06.2018 VEvent location Skalský dvůr Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords quantitative imaging ; Monte Carlo Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering OECD category Nano-materials (production and properties) R&D Projects GA17-15451S GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000450591400029 Annotation Labeling of specimens by nanoscale probes is common approach of complex biological
systems exploration. Namely gold nanoparticles immuno-staining is well established method
in electron microscopy. However, if more than two label sizes are used, the differentiation of
individual nanoparticles becomes difficult.
This can be overcome by cathodoluminescence (CL) active particles – nanophosphors where
labels recognition is done by wavelength of emitted light. This gives a great opportunity to
use advanced multi probe labeling within one sample.
There is a huge variety of nanophosphors: green fluorescent protein, quantum dots, ZnO
nanoparticles, organic molecules, rare earth-doped nanophosphors etc. Therefore, in order
to choose best type of nanophosphors for a given task, it is important to measure particles
size/thickness, as the CL intensity is proportional to the probe volume.Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2019
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