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Cytokinin signalling regulates organ identity via the AHK4 receptor in Arabidopsis
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SYSNO ASEP 0493505 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytokinin signalling regulates organ identity via the AHK4 receptor in Arabidopsis Author(s) Pernisová, M. (CZ)
Grochová, M. (CZ)
Konečný, T. (CZ)
Plačková, Lenka (UEB-Q) ORCID, RID
Haruštiaková, D. (CZ)
Kakimoto, T. (JP)
Heisler, M. (US)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Hejátko, J. (CZ)Number of authors 9 Article number dev163907 Source Title Development. - : Company of Biologists - ISSN 0950-1991
Roč. 145, č. 14 (2018)Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords lateral root primordia ; to-asp phosphorelay ; shoot meristem ; response regulators ; cell-differentiation ; transcription factor ; beta-glucosidase ; tissue-culture ; auxin efflux ; stem-cells ; Arabidopsis ; Cytokinin metabolism ; Cytokinin signalling ; De novo organogenesis ; Root ; Shoot Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LO1204 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000440421300010 EID SCOPUS 85050750952 DOI 10.1242/dev.163907 Annotation Mutual interactions of the phytohormones, cytokinins and auxin determine root or shoot identity during postembryonic de novo organogenesis in plants. However, our understanding of the role of hormonal metabolism and perception during early stages of cell fate reprogramming is still elusive. Here we show that auxin activates root formation, whereas cytokinins mediate early loss of the root identity, primordia disorganisation and initiation of shoot development. Exogenous and endogenous cytokinins influence the initiation of newly formed organs, as well as the pace of organ development. The process of de novo shoot apical meristem establishment is accompanied by accumulation of endogenous cytokinins, differential regulation of genes for individual cytokinin receptors, strong activation of AHK4-mediated signalling and induction of the shoot-specific homeodomain regulator WUSCHEL. The last is associated with upregulation of isopentenyladenine-type cytokinins, revealing higher shoot-forming potential when compared with trans-zeatin. Moreover, AHK4-controlled cytokinin signalling negatively regulates the root stem cell organiser WUSCHEL RELATED HOMEOBOX 5 in the root quiescent centre. We propose an important role for endogenous cytokinin biosynthesis and AHK4-mediated cytokinin signalling in the control of de novo-induced organ identity. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2019
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