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Bioaugmentation of PAH-contaminated soils: A novel procedure for introduction of bacterial degraders into contaminated soil
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SYSNO ASEP 0493333 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Bioaugmentation of PAH-contaminated soils: A novel procedure for introduction of bacterial degraders into contaminated soil Author(s) Innemanová, P. (CZ)
Filipová, Alena (MBU-M) RID, ORCID
Michalíková, Klára (MBU-M) ORCID
Wimmerová, L. (CZ)
Cajthaml, Tomáš (MBU-M) RID, ORCIDSource Title Ecological Engineering. - : Elsevier - ISSN 0925-8574
Roč. 118, AUG 1 (2018), s. 93-96Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords Polycyclic aromatic hydrocarbons ; Bioaugmentation ; Soil ; Biodegradation Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects TA01020106 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support MBU-M - RVO:61388971 UT WOS 000432518800010 EID SCOPUS 85046121664 DOI 10.1016/j.ecoleng.2018.04.014 Annotation An innovative method of inoculation of contaminated soils tested under laboratory conditions resulted in increased efficiency of PAH biodegradation in an aged industrial soil by almost 40% compared to biostimulation alone. The process encompassed inoculation of a small amount of contaminated soil with exogenous degraders. This first step was followed by a necessary propagation period of the introduced microbes. The material was further used as the inoculant for next step of the bioaugmentation procedure for a larger amount of contaminated soil in various ratios from 1:200 to 1:1000 and the process was repeated once more. The results showed that even the most diluted soil sample was very successful in terms of biodegradation of PAHs, resulting in 72.9% removal of PAHs including substantial biodegradation of heavy 4-5 ring PAHs. The employed next generation sequencing method provided evidence that one of the bioaugmentation strains, Acinetobacter calcoaceticus, was the dominant bacterial species in the soil and the results documented its involvement in the successful bioremediation experiment. The novel bioaugmentation method seems to be a promising strategy avoiding complications connected with the large-scale production of inoculant in bioreactors. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
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