Hybrid thermoresponsive graft constructs of fungal polysaccharide .beta.-glucan: physico-chemical and immunomodulatory properties

Loukotová, Lenka; Konefal, Rafal; Venclíková, Kristýna; Machová, Daniela; Janoušková, Olga; Rabyk, Mariia; Netopilík, Miloš; Mázl Chánová, Eliška; Štěpánek, Petr; Hrubý, Martin

doi:https://dx.doi.org/10.1016/j.eurpolymj.2018.07.004

Number of the records: 1

Hybrid thermoresponsive graft constructs of fungal polysaccharide .beta.-glucan: physico-chemical and immunomodulatory properties

1.

SYSNO ASEP	0491333
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Hybrid thermoresponsive graft constructs of fungal polysaccharide .beta.-glucan: physico-chemical and immunomodulatory properties
Author(s)	Loukotová, Lenka (UMCH-V)_{RID, ORCID} Konefal, Rafal (UMCH-V)_{RID, ORCID} Venclíková, Kristýna (UMCH-V)_RID Machová, Daniela (UMCH-V) Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Rabyk, Mariia (UMCH-V)_{RID, ORCID} Netopilík, Miloš (UMCH-V)_RID Mázl Chánová, Eliška (UMCH-V)_RID Štěpánek, Petr (UMCH-V)_{RID, ORCID} Hrubý, Martin (UMCH-V)_{RID, ORCID}
Source Title	European Polymer Journal. - : Elsevier - ISSN 0014-3057 Roč. 106, September (2018), s. 118-127
Number of pages	10 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	beta-glucan ; polyoxazoline ; multimodal cancer therapy
Subject RIV	CF - Physical ; Theoretical Chemistry
OECD category	Physical chemistry
R&D Projects	GA16-02870S GA ČR - Czech Science Foundation (CSF)
	GA16-03156S GA ČR - Czech Science Foundation (CSF)
	NV15-25781A GA MZd - Ministry of Health (MZ)
	7AMB16FR042 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMCH-V - RVO:61389013
UT WOS	000445993400014
EID SCOPUS	85049771016
DOI	10.1016/j.eurpolymj.2018.07.004
Annotation	We describe a family of thermoresponsive hybrid biodegradable peptidoglycan-like polymers, beta-glucan-graft-poly(2-isopropyl-2-oxazoline-co-2-butyl-2-oxazoline)s, in the context of their physical, chemical and biological properties. Four polymer samples, differing in the polyoxazoline graft length (500–5000 Da), were synthesized using beta-glucan extracted from Auricularia auricula-judae, which is known for its anticancer and immunomodulatory activities. The thermoresponsive behaviour of the prepared polymers were thoroughly studied using dynamic light scattering, fluorescence and magnetic resonance measurements, demonstrating an easy adjustment of the cloud point temperature by the graft length. Moreover, all samples show nontoxicity in vitro. The assay of oxidative burst of the polymorphonuclears established that the polymer immunostimulatory properties are not significantly influenced by their graft length considering the same amount of beta-glucan. Thus, we showed that the desired immunostimulatory properties are not decreased by the polyoxazoline grafting of β-glucan, even they are not influenced by the graft length of the polyoxazoline chains. Therefore, for the intended application the final graft length can be chosen considering the polymer thermoresponsive properties only, while not influencing their immunostimulatory properties.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2019

Number of the records: 1