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Biopolymer of Dictyosphaerium chlorelloides chemical characterization and biological effects
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SYSNO ASEP 0490105 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biopolymer of Dictyosphaerium chlorelloides chemical characterization and biological effects Author(s) Halaj, M. (SK)
Paulovičová, E. (SK)
Paulovicova, L. (SK)
Jantová, S. (SK)
Cepák, Vladislav (BU-J) RID
Lukavský, Jaromír (BU-J) RID, ORCID
Capek, P. (SK)Number of authors 7 Source Title International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 113, JUL 1 2018 (2018), s. 1248-1257Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Green microalgae ; Extracellular polysaccharide ; Cytokines Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects TE01020080 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Institutional support BU-J - RVO:67985939 CEZ AV0Z60050516 - BU-J (2005-2011) UT WOS 000432503100142 EID SCOPUS 85044147188 DOI 10.1016/j.ijbiomac.2018.03.052 Annotation Some microalgae are producers of high-value phytochemicals of interest for industrial applications. Cultivation of Dictyosphaerium chlorelloides microalgae yielded about 1 g/L of a viscous exobiopolymer with a high molecular weight (M-p, = 9.6 x 10(5) g/mol). Its proteoglycan character was confirmed by the presence of proteins and carbohydrates. Dominant sugars of the biopolymer were galactose and its methyl-derivatives (similar to 42 wt%), rhamnose (18 wt%), mannose (14 wt%) and unidentified methyl-hexoses (12 wt%). Besides, glucose and 3-0-methyl derivative (8 wt%), xylose and 4-0-methyl-derivative (4 wt%), and arabinose (2 wt%) were found. Methylation analysis indicated a complex chemical structure. Immunobiological studies revealed the effective anti-proliferative capacity of the biopolymer in vitro in both cancer and non-cancer cell lines. Based on in vitro exposition using a reconstructed 3-D tissue model, a good bioavailability and a low toxicity of the biopolymer have been found. Exposition of RAW 264.7 macrophages to the biopolymer resulted into significant immunostimulation of pro and anti-inflammatory cytokines, and decreased free radical cell release. Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2019
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