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Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome

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    SYSNO ASEP0489197
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleAnalysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome
    Author(s) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
    Pataridis, Statis (FGU-C)
    Eckhardt, Adam (FGU-C) RID, ORCID
    Sedmera, David (FGU-C) RID, ORCID, SAI
    Source TitleProtein Journal. - : Springer - ISSN 1572-3887
    Roč. 37, č. 1 (2018), s. 21-37
    Number of pages17 s.
    Languageeng - English
    CountryUS - United States
    Keywordsproteins of eggshell ; eggshell pigment ; crocodile
    Subject RIVCB - Analytical Chemistry, Separation
    OECD categoryAnalytical chemistry
    R&D ProjectsGA15-01948S GA ČR - Czech Science Foundation (CSF)
    GA16-02972S GA ČR - Czech Science Foundation (CSF)
    Institutional supportFGU-C - RVO:67985823
    UT WOS000426949100004
    EID SCOPUS85034666012
    DOI10.1007/s10930-017-9750-x
    AnnotationThe proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs).
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2019
Number of the records: 1  

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