Number of the records: 1
Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome
- 1.
SYSNO ASEP 0489197 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome Author(s) Mikšík, Ivan (FGU-C) RID, ORCID, SAI
Pataridis, Statis (FGU-C)
Eckhardt, Adam (FGU-C) RID, ORCID
Sedmera, David (FGU-C) RID, ORCID, SAISource Title Protein Journal. - : Springer - ISSN 1572-3887
Roč. 37, č. 1 (2018), s. 21-37Number of pages 17 s. Language eng - English Country US - United States Keywords proteins of eggshell ; eggshell pigment ; crocodile Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA15-01948S GA ČR - Czech Science Foundation (CSF) GA16-02972S GA ČR - Czech Science Foundation (CSF) Institutional support FGU-C - RVO:67985823 UT WOS 000426949100004 EID SCOPUS 85034666012 DOI 10.1007/s10930-017-9750-x Annotation The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs). Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2019
Number of the records: 1