Lipidomic analysis of Botryococcus (Trebouxiophyceae, Chlorophyta) - Identification of lipid classes containing very long chain fatty acids by offline two-dimensional LC-tandem MS

0488891 - MBÚ 2019 RIV GB eng J - Journal Article
Řezanka, Tomáš - Lukavský, Jaromír - Vítová, Milada - Nedbalová, L. - Sigler, Karel
Lipidomic analysis of Botryococcus (Trebouxiophyceae, Chlorophyta) - Identification of lipid classes containing very long chain fatty acids by offline two-dimensional LC-tandem MS.
Phytochemistry. Roč. 148, APR2018 (2018), s. 29-38. ISSN 0031-9422. E-ISSN 1873-3700
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971 ; RVO:67985939
Keywords : Green alga * Botryococcus * Botryosphaerella
OECD category: Microbiology
Impact factor: 2.905, year: 2018

Very long chain fatty acids (VLCFAs) were identified in four strains of the green alga Botryococcus braunii (Trebouxiophyceae). The algae contained a series of monoenoic fatty acids up to triacontenoic acid and further VLCFAs in amounts around 1% of total fatty acids. The separation of lipid classes using hydrophilic interaction chromatography revealed that the most abundant VLCFAs (28:2, 28:1 and 28:0) were contained in neutral lipids (triacylglycerols and/or diacylglycerols) and in phospholipids (phosphatidic acid and/or phosphatidylcholine). Using non-aqueous reversed-phase liquid chromatography tandem mass spectrometry (NARP-LC/MS2) of the appropriate collected fractions, molecular species of triacylglycerols containing one or two VLCFAs were described and phosphatidylcholines containing VLCFAs were separated for the first time. Because the presence of Botryosphaerella sudetica (Chlorophyceae) as contaminant of Botryococcus braunii strain Droop 1950/807-1 placed some doubts on the results of previous studies, a strain of this green alga of was also analyzed. In contrast to Botryococcus, C16, a substantially lower proportion of C18 polyunsaturated fatty acids and no VLCFAs were detected in Botryosphaerella.
Permanent Link: http://hdl.handle.net/11104/0283411