Degradation of phospholipids under different types of irradiation and varying oxygen saturation

0487625 - FZÚ 2018 RIV DE eng J - Journal Article
Vyšín, Luděk - Tomanová, K. - Pavelková, T. - Wagner, Richard - Davídková, Marie - Múčka, V. - Čuba, V. - Juha, Libor
Degradation of phospholipids under different types of irradiation and varying oxygen saturation.
Radiation and Environmental Biophysics. Roč. 56, č. 3 (2017), s. 241-247. ISSN 0301-634X. E-ISSN 1432-2099
R&D Projects: GA ČR GA13-28721S; GA ČR(CZ) GBP108/12/G108; GA MŠMT LM2015056
Institutional support: RVO:68378271 ; RVO:61389005
Keywords : lipid peroxidation * DOPC * gamma rays * eectrons * protons * oxygen saturation
OECD category: Biophysics
Impact factor: 1.527, year: 2017

The effects of different types of radiation on the formation of peroxide forms of 2-dioleoyl-sn-glycero-3-phosphocholine were studied under various conditions. For the irradiation, an aqueous solution of small unilamellar vesicles was prepared. Variations in parameters such as the dose rate and molecular oxygen saturation levels were evaluated. Our study suggests that the mechanism of the peroxides formation process remains unchanged under irradiation by accelerated electrons, gamma and accelerated protons. The values of radiation chemical yields of the peroxidic form depend on the type of radiation, dose rate, and the saturation of molecular oxygen. The level of oxygen saturation strongly affects the values of radiation chemical yields as well, as the dissolved oxygen is an important agent participating in peroxidation and it is a source of free radicals during the radiolysis. The values of radiation chemical yields strongly suggest that the mechanism of radiation-induced peroxidation of phosphatidylcholines does not proceed via chain reaction.
Permanent Link: http://hdl.handle.net/11104/0282272