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Stereology, an unbiased methodological approach to study plant anatomy and cytology: Past, present and future
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SYSNO ASEP 0484730 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Stereology, an unbiased methodological approach to study plant anatomy and cytology: Past, present and future Author(s) Kubínová, Lucie (FGU-C) RID, ORCID
Radochová, Barbora (FGU-C) RID, ORCID
Lhotáková, Z. (CZ)
Kubínová, Z. (CZ)
Albrechtová, J. (CZ)Source Title Image Analysis and Stereology. - : Društvo za stereologijo in kvantitativno analizo slike - ISSN 1580-3139
Roč. 36, č. 3 (2017), s. 187-205Number of pages 19 s. Language eng - English Country SI - Slovenia Keywords chloroplast ; confocal microscopy ; leaf anatomy ; mesophyll ; stereological methods ; systematic uniform random sampling Subject RIV FS - Medical Facilities ; Equipment OECD category Medical laboratory technology (including laboratory samples analysis R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 UT WOS 000423793500004 EID SCOPUS 85038411853 DOI 10.5566/ias.1848 Annotation This review presents an historical overview of stereological methods used for the quantitative evaluation of plant anatomical and cytological structures. It includes the origins of these methods up to the most recent developments such as the application of stereology based on 3D images. We focus especially on leaf, as the vast majority of studies of plant microscopic structure examine this organ. An overview of plant cell ultrastructure measurements as well as plant anatomical characteristics (e.g. plant tissue volume density, internal leaf surface area, number and mean size of mesophyll cells and chloroplast number), which were estimated by stereological methods most frequently, is presented. We emphasize the importance of proper sampling needed for unbiased measurements. Furthermore, we mention other methods used for plant morphometric studies and briefly discuss their relevance, precision, unbiasedness and efficiency in comparison with unbiased stereology. Finally, we discuss reasons for the sparse use of stereology in plant anatomy and consider the future of stereology in plant research. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2018
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