TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons

Kalynovska, Nataliia; Adámek, Pavel; Paleček, Jiří

doi:https://dx.doi.org/10.33549/physiolres.933613

Number of the records: 1

TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons

1.

SYSNO ASEP	0476981
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	TRPV1 receptors contribute to mediate paclitaxel-induced c-Fos expression in spinal cord dorsal horn neurons
Author(s)	Kalynovska, Nataliia (FGU-C)_{ORCID, RID} Adámek, Pavel (FGU-C)_{RID, ORCID, SAI} Paleček, Jiří (FGU-C)_{RID, ORCID}
Source Title	Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408 Roč. 66, č. 3 (2017), s. 549-532
Number of pages	4 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	c-Fos ; paclitaxel ; TRPV1 ; neuropathy ; spinal cord
Subject RIV	FH - Neurology
OECD category	Neurosciences (including psychophysiology
R&D Projects	GA15-11138S GA ČR - Czech Science Foundation (CSF)
	LH15279 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	FGU-C - RVO:67985823
UT WOS	000405692500019
EID SCOPUS	85026387474
DOI	10.33549/physiolres.933613
Annotation	Transient receptor potential vanilloid type 1 (TRPV1) receptors are important in the development of different pathological chronic pain states. Here we examined the role of spinal cord TRPV1 receptors in the mechanisms leading to activation of dorsal horn neurons after paclitaxel (PAC) treatment. PAC is a widely used chemotherapeutic drug that often leads to development of painful neuropathy. Immunohistochemical analysis of c-Fos protein expression in dorsal horn neurons was used as a marker of neuronal activation. Rat spinal cord slices were processed for in vitro incubation with PAC (100 nM) and TRPV1 receptor antagonists (SB366791 and AMG9810, 10 mikroM). PAC treatment induced significant upregulation of c-Fos nuclear expression in superficial dorsal horn neurons that was diminished by TRPV1 receptor antagonists pre-incubation. These results further substantiated the role of spinal TRPV1 receptors in the development of paclitaxel-induced neuropathic pain and contribute to better understanding of the pathological mechanisms involved.
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2018

Number of the records: 1