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An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes
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SYSNO ASEP 0473595 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title An advanced conjugation strategy for the preparation of quantum dot-antibody immunoprobes Author(s) Dvořáková, V. (CZ)
Čadková, M. (CZ)
Datinská, Vladimíra (UIACH-O)
Klepárník, Karel (UIACH-O) RID, ORCID
Foret, František (UIACH-O) RID, ORCID
Bílková, Z. (CZ)
Korecká, L. (CZ)Number of authors 7 Source Title Analytical Methods: advancing methods and applications . - : Royal Society of Chemistry - ISSN 1759-9660
Roč. 9, č. 13 (2017), s. 1991-1997Number of pages 7 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords quantum dots ; immunoprobes ; antibodies Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA17-01995S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000399912500003 EID SCOPUS 85016457050 DOI 10.1039/c6ay03322a Annotation An advanced site-specific conjugation strategy for the preparation of quantum dot-based antibody probes applicable in various immunoassays from fluorescence to electrochemical biosensors is described. The combination of antigen (protein ApoE) modified magnetic particles providing protection to antibody binding sites, simple carbodiimide chemistry and carboxylate quantum dots (QDs) made of CdSe/ZnS is used for efficient labelling of anti-ApoE antibodies representing a model system. Polyacrylamide gel electrophoresis, fluorescence spectra measurements, capillary electrophoresis-laser induced fluorescence (CE-LIF) and square wave anodic stripping voltammetry (SWASV) were used for experimental verification of labelling efficiency. A simple change in antibody type makes this approach versatile and exploitable in a wide range of applications. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2018
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