Number of the records: 1  

Effect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats

  1. 1.
    SYSNO ASEP0470081
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleEffect of Erythropoietin, Iron Deficiency and Iron Overload on Liver Matriptase-2 (TMPRSS6) Protein Content in Mice and Rats
    Author(s) Frýdlová, J. (CZ)
    Přikryl, P. (CZ)
    Truksa, Jaroslav (BTO-N) RID, ORCID
    Falke, L. L. (NL)
    Du, X. (US)
    Gurieva, I. (CZ)
    Vokurka, M. (CZ)
    Krijt, J. (CZ)
    Number of authors8
    Article numbere0148540
    Source TitlePLoS ONE. - : Public Library of Science - ISSN 1932-6203
    Roč. 11, č. 2 (2016)
    Number of pages17 s.
    Languageeng - English
    CountryUS - United States
    KeywordsHEPCIDIN EXPRESSION ; SERINE-PROTEASE ; GENE-EXPRESSION
    Subject RIVEB - Genetics ; Molecular Biology
    R&D ProjectsGA13-28830S GA ČR - Czech Science Foundation (CSF)
    ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportBTO-N - RVO:86652036
    UT WOS000369552800066
    DOI10.1371/journal.pone.0148540
    AnnotationMatriptase-2 (TMPRSS6) is an important negative regulator of hepcidin expression; however, the effects of iron overload or accelerated erythropoiesis on liver TMPRSS6 protein content in vivo are largely unknown. We determined TMPRSS6 protein content in plasma membrane-enriched fractions of liver homogenates by immunoblotting, using a commercial antibody raised against the catalytic domain of TMPRSS6. Plasma membrane-enriched fractions were obtained by centrifugation at 3000 g and washing. TMPRSS6 was detected in the 3000 g fraction as a 120 kDa full-length protein in both mice and rats. Feeding of iron-deficient diet as well as erythropoietin treatment increased TMPRSS6 protein content in rats and mice by a posttranscriptional mechanism; the increase in TMPRSS6 protein by erythropoietin was also observed in Bmp6-mutant mice. Administration of high doses of iron to mice (200, 350 and 700 mg/kg) decreased TMPRSS6 protein content. Hemojuvelin was detected in the plasma membrane-enriched fractions of control animals as a full length protein of approximately 52 kDa; in iron deficient animals, the full length protein was partially cleaved at the N-terminus, resulting in an additional weak band of approximately 47 kDa. In livers from hemojuvelin-mutant mice, TMPRSS6 protein content was strongly decreased, suggesting that intact hemojuvelin is necessary for stable TMPRSS6 expression in the membrane. Overall, the results demonstrate posttranscriptional regulation of liver TMPRSS6 protein by iron status and erythropoietin administration, and provide support for the interaction of TMPRSS6 and hemojuvelin proteins in vivo.
    WorkplaceInstitute of Biotechnology
    ContactMonika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700
    Year of Publishing2017
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.