DNA and chromosomal damage in medical workers exposed to anaesthetic gases assessed by the lymphocyte cytokinesis-block micronucleus (CBMN) assay. A critical review

0469100 - ÚEM 2017 RIV NL eng J - Journal Article
Vodička, Pavel - Mušák, L. - Fiorito, G. - Vymetálková, Veronika - Vodičková, Ludmila - Naccarati, Alessio
DNA and chromosomal damage in medical workers exposed to anaesthetic gases assessed by the lymphocyte cytokinesis-block micronucleus (CBMN) assay. A critical review.
Mutation Research-Reviews in Mutation Research. Roč. 770, SI (2016), s. 26-34. ISSN 1383-5742. E-ISSN 1388-2139
R&D Projects: GA MŠMT(CZ) LD14050; GA ČR(CZ) GA15-14789S
Institutional support: RVO:68378041
Keywords : micronuclei * anaesthetics gas exposure * operating room personnel
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.500, year: 2016

The lymphocyte cytokinesis-block micronucleus (CBMN) assay has been applied in hundreds of in vivo biomonitoring studies of humans exposed either environmentally or occupationally to genotoxic chemicals. However, there is an emerging need to re-evaluate the use of MN and other biomarkers within the lymphocyte CBMN cytome assay as quantitative indicators of exposure to main classes of chemical genotoxins.

The main aim of the present report is to systematically review published studies investigating the use of the lymphocyte CBMN assay to determine DNA damage in subjects exposed to anaesthetic gases. We also compared performance of the CBMN assay with other DNA damage assays employed and identified strengths and weaknesses of the published studies. We have retrieved 11 studies, published between 1996 and 2013, reporting MN associated with occupational exposures (operating room personnel). The individual job categories were often described (anaesthesiologists, technicians, radiologists) among cases, as well as duration of exposure. All studies reported the compounds present at the workplace and, in some instances, the exposure levels Were measured. Controls were usually recruited among personnel at the hospital not exposed to anaesthetics or they were healthy unexposed subjects from general population. The number of investigated subjects, due to the character of the occupation, was relatively smaller than those investigated in other occupational monitoring settings. Overall, the majority of the studies were age- and gender- matched (or investigated only males or females) while less attention was given to lifestyle confounders. Appropriate measurement of exposure, available in approximately half of the studies only, was compromised by the lack of the personal dosimetry-based determinations.

Permanent Link: http://hdl.handle.net/11104/0266981