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Expression of estrogene receprot beta in the male reproductive tract and sperm in mice
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SYSNO ASEP 0463169 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Expression of estrogene receprot beta in the male reproductive tract and sperm in mice Author(s) Dostálová, Pavla (BTO-N)
Dorosh, Andriy (BTO-N) RID
Elzeinová, Fatima (BTO-N) RID
Děd, Lukáš (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)
Pěknicová, Jana (BTO-N) RIDNumber of authors 6 Source Title Abstract book of the 48th Annual Meeting of the Society for the Study of Reproduction. - San Juan : Society for the Study of Reproduction, 2015 Number of pages 1 s. Action 48th Annual Meeting of the Society for the Study of reproduction Event date 18.06.2015-22.06.2015 VEvent location San Juan Country US - United States Event type WRD Language eng - English Country US - United States Keywords estrogen receptor beta ; estrogen receptor alpha ; elutriation ; gene expression Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA14-05547S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Annotation Estrogens are sex steroid hormones that play an important role in male reproduction. Generally, estrogen action is mediated via binding and activation of estrogen receptors (ERs). Activated ERs form dimers, bind to DNA and act as transcription factors. Estrogen binding can also lead to rapid activation of several signalling cascades. Currently, two types of classical ERs are known, namely ER alpha and ER beta. In addition to classical ERs, several splice variants that may differ in their ligand- or DNA-binding properties were detected. These variants may also form dimers and modulate the function of classical full-length ERs. Estrogen action seems to be a very complex and therefore it is necessary to known the exact expression pattern of all existing variants of ERs. Since ERβ is a predominant variant in testes, we focused our study on expression of ERβ and its potential splice variants in murine testes, sperm and epididymis. According to our results from mRNA analysis, there are at least two variants of ERβ in mice testes, epididymis and sperm. These variants differ in one exon in ligand binding domain and this variability leads to different affinity to estrogens. It seems that both variants are similar in abundance within the same organ or sperm. Furthermore, cauda epididymis and sperm from cauda epididymis contain fewer ERβ compared to caput epididymis and sperm from this region. To analyse these variants also at a protein level, we prepared specific monoclonal antibodies recognizing particular variant of ERβ. Both antibodies detected band(s) in protein extracts from testes and epididymis. The present study demonstrated that at least two variants of ERβ are expressed in male reproductive tract. Further studies are needed to determine whether these variants are present within a single cell and can thus interact, or whether one type of cells preferentially express one ERβ variant while another cell type express the second variant. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
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