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Study of sperm proteins in different mammalian species
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SYSNO ASEP 0463007 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type The record was not marked in the RIV Title Study of sperm proteins in different mammalian species Author(s) Pohlová, Alžběta (BTO-N)
Zigo, Michal (BTO-N) RID
Jonáková, Věra (BTO-N) RID
Postlerová, Pavla (BTO-N) ORCID, RIDNumber of authors 4 Source Title Book of abstracts of XXIInd Symposium of biology and immunology of reproduction. - Vestec u Prahy : Biotechnologický ústav AVČR, 2016 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 29-29Number of pages 1 s. Action XXIInd Symposium of biology and immunology of reproduction with international participation Event date 26.05.2016-28.05.2016 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Keywords sperm surface proteins ; glycoproteins profiles ; sperm - zona pellucida interaction ; monoclonal antibodies Subject RIV CE - Biochemistry R&D Projects GA 14-05547S GA ČR - Czech Science Foundation (CSF) Institutional support BTO-N - RVO:86652036 Annotation Reproduction is an essential feature of all animals and a fundamental step to produce new generations. Study of sperm proteins is crucial for understanding of the sperm-egg recognition. We searched out sperm surface proteins involving in the zona pellucida (ZP) binding and studied whether these proteins are preserved throughout mammalian species. Indirect immunofluorescent technique was used to test a panel of monoclonal antibodies prepared against boar sperm surface proteins on spermatozoa of bull and mice. We found a cross-reactivity of some antibodies against boar sperm with bull ejaculated and mouse epididymal spermatozoa. Further, we isolated sperm proteins from different mammalian species, such as pig, bull, dog, cat, mouse and human. Proteins were separated by SDS-electrophoresis and protein/glycoprotein profiles from epididymal, ejaculated and in vitro capacitated sperm were compared. The interaction of sperm with ZP was studied on electrophoretically-separated sperm surface proteins from pig and bull with biotin-labeled ZP glycoproteins using Far Western blot. Antibodies, which reacted with boar sperm surface proteins with ZP-binding activity, therefore could be potential egg-binding receptors, were used for monitoring of the sperm protein origin in reproductive fluids and tissues. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
Number of the records: 1