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Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm
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SYSNO ASEP 0462995 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type The record was not marked in the RIV Title Possible role of spermatogenic protein glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) in mammalian sperm Author(s) Margaryan, Hasmik (BTO-N) RID
Dorosh, Andriy (BTO-N) RID
Čapková, Jana (BTO-N)
Postlerová, Pavla (BTO-N) ORCID, RID
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCID
Pěknicová, Jana (BTO-N) RIDNumber of authors 7 Source Title Book of abstracts of XXIst Symposium of biology and immunology of reproduction. - Praha : Biotechnologický ústav AVČR, 2015 / Pěknicová Jana ; Elzeinová Fatima ; Kubátová Alena
S. 31-31Number of pages 1 s. Action XXIst SYMPOSIUM OF BIOLOGY AND IMMUNOLOGY OF REPRODUCTION WITH INTERNATIONAL PARTICIPATION Event date 14.05.2015-16.05.2015 VEvent location Třešť Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords GAPDHS ; sperm glycolytic enzyme ; zona pellucida glycoprotein matrix ; monoclonal antibody Subject RIV CE - Biochemistry R&D Projects GA P503/12/1834 GA ČR - Czech Science Foundation (CSF) Annotation Sperm proteins are important for the structure and function of these specific, highly differentiated cells. Certain of these proteins play a role in sperm-egg recognition during primary or secondary binding at zona pellucida glycoprotein matrix. The aim of this study was to characterize the acrosomal sperm protein recognized by a monoclonal antibody (MoAb) Hs-8, prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperm, and to test the possible role of this protein in gamete interaction. MoAb Hs-8 specifically labelled a 45 kDa protein from the sperm extract in the immunoblotting test. Sequence analysis identified this Hs-8 protein as GAPDHS. In order to perform a control tests, a commercial mouse anti-GAPDHS MoAb was applied. Both antibodies showed similar staining patterns using immunofluorescence labelling, transmission electron microscopy and immunoblot analysis. Moreover, both Hs-8 and commercial anti-GAPDHS antibodies blocked the secondary sperm-zona pellucida binding. Generally, GAPDHS was considered mainly as sperm-specific glycolytic enzyme involved in energy production during spermatogenesis and sperm motility and its role in the sperm head was unknown. In this study, we confirmed the potential additional role of GAPDHS as a binding protein that is involved in the sperm-zona pellucida interaction. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
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