Technical note: Efficient removal of a PCR inhibitory agent (vivianite) found on excavated bones

0462667 - ARÚ 2017 RIV IE eng J - Journal Article
Votrubová-Dubská, J. - Vaněk, D. - Zikmund, J. - Mestek, O. - Urbanová, V. - Brzobohatá, Hana - Brestovanský, P.
Technical note: Efficient removal of a PCR inhibitory agent (vivianite) found on excavated bones.
Forensic Science International. Roč. 261, April (2016), s. 8-13. ISSN 0379-0738. E-ISSN 1872-6283
R&D Projects: GA ČR GB14-36938G
Institutional support: RVO:67985912
Keywords : vivianite * PCR inhibitors * DNA extraction
Subject RIV: AC - Archeology, Anthropology, Ethnology
Impact factor: 1.989, year: 2016

During a rescue excavation in October 2011, archaeologists discovered a mass grave with 10 individuals. The skeletons should belong to victims of the battle of Reichenberg between the Austrian and Prussian armies on April 21, 1757. Several bones of the skeletons were covered with a blue colored encrustation. Initial DNA analysis failed due to strong inhibition. Chemical analysis of the bluish encrustation indicated the presence of the iron phosphate mineral vivianite (Fe3(PO4)2.(H2O)8). This technical note describes a novel procedure for the removal of this inhibitory substance.
Permanent Link: http://hdl.handle.net/11104/0262074