G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques

0456709 - BFÚ 2016 RIV DE eng J - Journal Article
Vidláková, Pavlína - Pivoňková, Hana - Kejnovská, Iva - Trnková, L. - Vorlíčková, Michaela - Fojta, Miroslav - Havran, Luděk
G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques.
Analytical and Bioanalytical Chemistry. Roč. 407, č. 19 (2015), s. 5817-5826. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA ČR GAP206/12/2378
Institutional support: RVO:68081707
Keywords : Oligonucleotides * Electrochemical methods * G-quadruplex
Subject RIV: BO - Biophysics
Impact factor: 3.125, year: 2015

Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG) (n) stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak G(ox)) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak G(HMDE) was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG) (n) stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues.
Permanent Link: http://hdl.handle.net/11104/0257223