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Microbiology Monographs
- 1.0426071 - MBÚ 2014 RIV DE eng M - Monography Chapter
Pátek, Miroslav - Nešvera, Jan
Promoters and Plasmid Vectors of Corynebacterium glutamicum.
Microbiology Monographs. Vol. 23. Berlin,Heidelberg: Springer-Verlag, 2013 - (Yukawa, H.; Inui, M.), s. 51-88. ISBN 978-3-642-29856-1
R&D Projects: GA ČR GC204/09/J015
Institutional support: RVO:61388971
Keywords : gene expression * Corynebacterium glutamicum
Subject RIV: EE - Microbiology, Virology
Various classes of C. glutamicum promoters, their specific functions in gene expression control, and methods for their analysis are described in this review. C. glutamicum promoters recognized by RNA polymerase with a specific sigma factor (SigA, SigB, SigH, or SigM) were localized and their consensus sequences determined. Experimental localization of transcriptional start points revealed complex gene expression regulation features, such as overlapping multiple promoters. An overview of native plasmids used for the construction of C. glutamicum vectors and examples of vectors applied in genetic analysis are provided. In addition to basic cloning vectors, special purpose vectors are described. This includes promoter-probe vectors for the analysis of promoter activity profiles and various regulatory mechanism and expression vectors, which carry promoters as efficient signals for gene expression.
Permanent Link: http://hdl.handle.net/11104/0231822
Number of the records: 1