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Identification of bifidobacteria isolated from Asian elephant (Elephas maximus)
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SYSNO ASEP 0425225 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of bifidobacteria isolated from Asian elephant (Elephas maximus) Author(s) Bunešová, V. (CZ)
Vlková, E. (CZ)
Rada, V. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Kmeť, V. (SK)Source Title Journal of Biosciences. - : Indian Academy of Sciences - ISSN 0250-5991
Roč. 38, č. 2 (2013), s. 239-243Number of pages 5 s. Publication form Print - P Language eng - English Country IN - India Keywords 16S rRNA genes sequencing ; bifidobacteria ; Elephas maximus Subject RIV EE - Microbiology, Virology R&D Projects GA523/08/1091 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000319153600006 EID SCOPUS 84878013463 DOI 10.1007/s12038-013-9322-y Annotation Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60 +/- 0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2014
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