Environmental scanning electron microscope for observation of live nature

0421179 - ÚPT 2014 JP eng C - Conference Paper (international conference)
Neděla, Vilém - Tihlaříková, Eva
Environmental scanning electron microscope for observation of live nature.
69th Annual Meeting of the Japanese Society of Microscopy. Osaka: The Japanese Society of Microscopy, 2013.
[Annual Meeting of the Japanese Society of Microscopy /69./. Osaka (JP), 20.05.2013-22.05.2013]
Institutional support: RVO:68081731
Keywords : ESEM * AQUASEM II * live nature * detectors
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

We have developed an ESEM for in-situ observation of live nature. Our AQUASEM II is base on a Tescan VEGA SEM. Its exceptionality is an original differentially-pumped chamber, which can be evacuated slowly and gently to the sample, and a specific detection system with four detectors. On the basis of our new methodology proved by the results of Monte Carlo simulations of beam electrons scattering in water, we have recorded the moving of a Stored-product mite alive for ~5 min at a vapor pressure of 950 Pa at 7oC. Our results demonstrate that this microscope is well applicable to in-situ observations of natural structure of biological or organic samples in wet modes, non-coated surfaces of materials such as semiconductors and oxides, growth or reaction processes of nanoparticles in solution, etc.
Permanent Link: http://hdl.handle.net/11104/0227599