FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion

Ostašov, Pavel; Sýkora, Jan; Brejchová, Jana; Olžyńska, Agnieszka; Hof, Martin; Svoboda, Petr

doi:https://dx.doi.org/10.1016/j.chemphyslip.2013.02.006

Number of the records: 1

FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion

1.

SYSNO ASEP	0391710
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion
Author(s)	Ostašov, Pavel (FGU-C) Sýkora, Jan (UFCH-W)_RID Brejchová, Jana (FGU-C)_{RID, ORCID} Olžyńska, Agnieszka (UFCH-W)_RID Hof, Martin (UFCH-W)_{RID, ORCID} Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084 167-168, FEB-MAR (2013), s. 62-69
Number of pages	8 s.
Language	eng - English
Country	IE - Ireland
Keywords	cholesterol depletion ; beta-Cyclodextrin ; 22-NBD-cholesterol ; 25-NBD-cholesterol ; FLIM studies ; intact HEK293 cells
Subject RIV	CE - Biochemistry
Subject RIV - cooperation	J. Heyrovsky Institute of Physical Chemistry - Physical ; Theoretical Chemistry
R&D Projects	GAP207/12/0919 GA ČR - Czech Science Foundation (CSF)
Institutional support	FGU-C - RVO:67985823 ; UFCH-W - RVO:61388955
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000318137600008
EID SCOPUS	84875334518
DOI	10.1016/j.chemphyslip.2013.02.006
Annotation	The observation that 22-NBD-cholesterol appears to be indifferent to the changes in the membrane packing in living cells is in agreement with previous studies in model membranes. However, our data indicate that the alternation of plasma membrane structure induced by decrease of cholesterol level by beta-CDX makes the membrane environment of NBD moiety of 25-NBD-cholesterol probe a significantly more hydrated. This finding not only encourages using 25-NBD-cholesterol in living cells, but also demonstrates that previously drawn discouraging conclusions on the use of 25-NBD-cholesterol in model membranes are not valid for living cells
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2014

Number of the records: 1