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Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin
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SYSNO ASEP 0391532 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Imaging of Protein Synthesis: In Vitro and In Vivo Evaluation of Sc-44-DOTA-Puromycin Author(s) Eigner, Sebastian (UJF-V) RID
Beckford, Denis R. (UJF-V) RID
Fellner, M. (DE)
Loktionova, N. (DE)
Piel, M. (DE)
Lebeda, Ondřej (UJF-V) RID, ORCID, SAI
Rosch, F. (DE)
Ross, T. L. (DE)
Eigner-Henke, Kateřina (UJF-V) RIDNumber of authors 9 Source Title Molecular Imaging and Biology. - : Springer - ISSN 1536-1632
Roč. 15, č. 1 (2013), s. 79-86Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords protein synthesis ; Scandium-44 ; DOTA-Pur ; therapy control ; mu PET ; preclinical imaging Subject RIV FR - Pharmacology ; Medidal Chemistry R&D Projects 2B06165 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UJF-V - RVO:61389005 UT WOS 000314033000010 DOI 10.1007/s11307-012-0561-3 Annotation he purpose of this study was to investigate whether Sc-44-labeled puromycin can be utilized for imaging of protein synthesis in vivo. For micro-positron emission tomographic (mu PET) studies, 20-25 MBq of [Sc-44]-DOTA-puromycin was administered to tumor-bearing rats, and animals were scanned for 1 h dynamically. Results were further validated by dissecting organs and tissues of the animals after the measurement and in vitro blocking experiments using puromycin or cycloheximide to block protein synthesis. mu PET images of tumor-bearing rats showed significant tumor uptake of [Sc-44]-DOTA-puromycin and a clear-cut tumor visualization. In both blocking experiments, cellular uptake of [Sc-44]-DOTA-puromycin ([Sc-44]-DOTA-Pur) could be suppressed by blocking protein synthesis. We report for the first time successful mu PET imaging with Sc-44 obtained from a Ti-44/Sc-44 generator, as well as noninvasive mu PET imaging of ribosomal activity, respectively protein synthesis, with a puromycin-based radiopharmaceutical and the direct correlation between cellular uptake of [Sc-44]-DOTA-Pur and protein synthesis. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2014 Electronic address http://link.springer.com/content/pdf/10.1007%2Fs11307-012-0561-3
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