Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis

0377469 - BC 2013 RIV NL eng J - Journal Article
Špak, Josef - Votruba, Ivan - Pavingerová, Daniela - Holý, Antonín - Špaková, Vlastimila - Petrzik, Karel
Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis.
Plant Cell, Tissue and Organ Culture. Roč. 10, č. 1 (2012), s. 63-68. ISSN 0167-6857. E-ISSN 1573-5044
R&D Projects: GA ČR GA522/09/0707
Institutional research plan: CEZ:AV0Z50510513; CEZ:AV0Z40550506
Keywords : Caulimovirus * Chemotherapy * Pararetrovirus * dsDNA
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.633, year: 2012

Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l -1 and PMEDAP at concentrations 50 and 12. 5 mg l -1 significantly (P < 0.05) reduced CaMV concentration in plants within 42-63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12. 5, 25 and 50 mg l -1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0. 05) already at 12. 5 mg l -1 with both compounds.
Permanent Link: http://hdl.handle.net/11104/0209617