The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic)

0375994 - BC 2013 RIV NL eng J - Journal Article
Němejc, K. - Sak, Bohumil - Květoňová, Dana - Hanzal, V. - Jeníková, Martina - Kváč, Martin
The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic).
Veterinary Parasitology. Roč. 184, 2/4 (2012), 122-125. ISSN 0304-4017. E-ISSN 1873-2550
Grant - others:Mšk(CZ) 6007665806
Institutional research plan: CEZ:AV0Z60220518; CEZ:AV0Z50450515
Keywords : Cryptosporidium suis * Cryptosporidium pig genotype II * Eurasian wild boar * SSU * PCR
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 2.381, year: 2012

A total of 193 faecal samples of adult Eurasian wild boars were collected at 12 enclosures across the Czech Republic and examined for Cryptosporidium infection using both microscopic and molecular tools. Cryptosporidium oocysts were not detected in any of the 193 faecal samples examined using the aniline-carbol-methyl violet staining method. Thirty-two positive cases of Cryptosporidium infection were detected using either genus-or species-specific nested PCR. Mono-infection with Cryptosporidium suis and Cryptosporidium pig genotype II were found in 13 and 7 cases, respectively. Five mixed infections of C suis and Cryptosporidium pig genotype II were detected using PCR/RFLP with genus specific primers. The number of detected mixed infections increased 2.4 fold when a species-specific PCR was employed. This is the first report on the Cryptosporidium infection caused by C suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa).
Permanent Link: http://hdl.handle.net/11104/0208515