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Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes
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SYSNO ASEP 0374555 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes Author(s) Pimková, K. (CZ)
Bocková, Markéta (URE-Y)
Hegnerová, Kateřina (URE-Y)
Suttnar, J. (CZ)
Čermák, J. (CZ)
Homola, Jiří (URE-Y) RID
Dyr, J. E. (CZ)Number of authors 7 Source Title Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 402, č. 1 (2012), s. 381-387Number of pages 7 s. Language eng - English Country DE - Germany Keywords surface plasmon resonance ; myelodysplastic syndromes ; vascular endothelial growth factor Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z20670512 - URE-Y (2005-2011) UT WOS 000298749300041 DOI 10.1007/s00216-011-5395-3 Annotation The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2013
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