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Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8(+) T-Cells
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SYSNO ASEP 0361425 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Breakdown of the Blood-Brain Barrier during Tick-Borne Encephalitis in Mice Is Not Dependent on CD8(+) T-Cells Author(s) Růžek, Daniel (BC-A) RID, ORCID
Salát, Jiří (BC-A) RID, ORCID
Singh, S. K. (IN)
Kopecký, Jan (BC-A) RIDSource Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 6, č. 5 (2011), e20472Number of pages 9 s. Language eng - English Country US - United States Keywords CENTRAL-NERVOUS-SYSTEM ; WEST-NILE-VIRUS ; MICROVASCULAR ENDOTHELIAL-CELLS ; TIGHT JUNCTION PROTEINS ; MULTIPLE-SCLEROSIS ; INFLAMMATORY RESPONSE ; PERMEABILITY CHANGES Subject RIV EB - Genetics ; Molecular Biology R&D Projects GPP302/10/P438 GA ČR - Czech Science Foundation (CSF) GAP502/11/2116 GA ČR - Czech Science Foundation (CSF) LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000291005200058 DOI 10.1371/journal.pone.0020472 Annotation Tick-borne encephalitis (TBE) virus causes severe encephalitis with serious sequelae in humans. The disease is characterized by fever and debilitating encephalitis that can progress to chronic illness or fatal infection. In this study, changes in permeability of the blood-brain barrier (BBB) in two susceptible animal models (BALB/c, and C57Bl/6 mice) infected with TBE virus were investigated at various days after infection by measuring fluorescence in brain homogenates after intraperitoneal injection of sodium fluorescein, a compound that is normally excluded from the central nervous system. We demonstrate here that TBE virus infection, in addition to causing fatal encephalitis in mice, induces considerable breakdown of the BBB. Breakdown of the BBB was also observed in mice deficient in CD8(+) T-cells, indicating that these cells are not necessary for the increase in BBB permeability that occurs during TBE. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2012
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