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New and simple staining method for visualizing UHMWPE lamellar structure in TEM
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SYSNO ASEP 0321134 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title New and simple staining method for visualizing UHMWPE lamellar structure in TEM Title Nová jednoduchá metoda zviditelnění lamelární struktury UHMWPE v TEM Author(s) Stará, Hana (UMCH-V) RID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Lednický, František (UMCH-V)
Pavlova, Ewa (UMCH-V) RID
Baldrian, Josef (UMCH-V)
Starý, Zdeněk (UMCH-V) RIDNumber of authors 6 Source Title Journal of Macromolecular Science - Physics - ISSN 0022-2348
Roč. 47, č. 6 (2008), s. 1148-1160Number of pages 13 s. Language eng - English Country US - United States Keywords UHMWPE ; polyethylene ; lamellar structure Subject RIV CD - Macromolecular Chemistry R&D Projects 2B06096 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000260431100011 DOI 10.1080/00222340802403263 Annotation Ultra-high-molecular-weight polyethylene (UHMWPE) is used in arthroplasty as a key component of total joint replacements. In this study we introduce a new staining method that makes it possible to visualize the UHMWPE lamellae by transmission electron microscopy (TEM). The method consists of one-step staining with oleum (H2SO4 solution of SO3) for 4 days and ultramicrotomy; this is significantly simpler than previously described techniques and yields micrographs of the same quality. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2009
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