Effect of various synthetic dyes on the production of manganese-dependent peroxidase isoenzymes by immobilized Irpex lacteus

0313101 - MBÚ 2009 RIV NL eng J - Journal Article
Šušla, Martin - Svobodová, Kateřina
Effect of various synthetic dyes on the production of manganese-dependent peroxidase isoenzymes by immobilized Irpex lacteus.
[Vliv syntetických barviv na produkci isoforem mangan-dependentní peroxidasy u imobilizované houby Irpex lacteus.]
World Journal of Microbiology and Biotechnology. Roč. 24, - (2008), s. 225-230. ISSN 0959-3993. E-ISSN 1573-0972
R&D Projects: GA ČR GP526/06/P102
Grant - others:CZ(CZ) G4 359/2007
Institutional research plan: CEZ:AV0Z50200510
Keywords : irpex lacteus * isoenzymes * manganese-dependent peroxidase
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.945, year: 2008

The effect of manganese and selected synthetic dyes on the production of manganese-dependent peroxidase (MnP) by Irpex lacteus immobilized on polyuretane foam was studied. In the cultures grown in a medium containing 65 µM Mn (II), up to three various isoenzymes of MnP were resolved by isolectrofocusing, with pI values within the range of 3.50–6.04. In the cultures grown in a medium containing 2.9 mM Mn (II), two new MnP isoforms (pI 3.28, 3.75) were produced. The addition of structurally different synthetic dyes, an azo dye Reactive Orange 16 (RO16), an anthraquinonic dye Remazol Brilliant Blue R (RBBR), and a triphenylmethane dye Bromophenol Blue (BPB), to the fungal cultures grown in the presence of high manganese inhibited the production of low pI MnP isoforms. However, in the presence of BPB a new MnP isoform with pI 5.67 was detected

Studován byl efekt syntetických barviv na produkci mangan peroxidasy houbou Irpex lacteus imobilizovanou na polyuretanové pěně. V kulturách s médiem obsahujícím 65 µM Mn (II) byly detekovány tři různé formy MnP o pI 3,5 – 6,04. V médiu s vysokým obsahem Mn (2.9 mM) byly produkovány dvě nové formy enzymu (pI 3.28, 3.75). Přidání syntetických barviv, Reactive Orange 16 (RO16), Remazol Brilliant Blue R (RBBR), a Bromophenol Blue (BPB), do média s vysokým obsahem Mn inhibovalo produkci MnP s nízkým pI. BPB navíc indukovala produkci zcela nové formy enzymu s pI=5,67. MnP produkované kulturami v přítomnosti BPB byly schopné účinnější dekolonizace RBBR než enzymy produkované v kontrolních kulturách
Permanent Link: http://hdl.handle.net/11104/0164015