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Bovine seminal ribonuclease exerts selective cytotoxicity toward neuroblastoma cells both sensitive and resistant to chemotherapeutic drugs
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SYSNO ASEP 0213245 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Bovine seminal ribonuclease exerts selective cytotoxicity toward neuroblastoma cells both sensitive and resistant to chemotherapeutic drugs Author(s) Činátl, J. (DE)
Činátl, J.jr. (DE)
Kotchetkov, R. (DE)
Matoušek, Josef (UZFG-Y) RID
Woodcock, B. G. (DE)
KOehl, U. (DE)
Vogel, J. U. (DE)
Kornhuber, B. (DE)
Schwabe, D. (DE)Source Title Anticancer Research. - : International Institute of Anticancer Research - ISSN 0250-7005
Roč. 20, - (2000), s. 853-860Number of pages 8 s. Language eng - English Country GR - Greece Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/96/1738 GA ČR - Czech Science Foundation (CSF) KSK2038602 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KSK2052601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation Background. Bovine seminal ribonuclease (BS-RNase) exerts selective cytotoxicity toward different types of tumor cells. In the present study, we tested the effects of BS-RNase on cultured neuroblastoma (NB) cells resistant to chemotherapeutic agents. The selectivity of the antitumoral activity of BS-RNase was evaluated using cultures of CD34(+) hematopoietic stem cells. Materials and Methods Human NE cell lines including IMR-32, UKF-NB-2 and UKF-NB-3 were selected for resistance against vincristine, doxorubicin or cisplatin by exposure to increasing concentrations of the respective drug. The cytotoxicity of the drugs to NE cells was evaluated using a clonogenic assay in a methylcellulose medium. Peripheral blood progenitor cells were obtained from adult healthy donors by positive selection using specific anti-CD34(+) antibodies. The toxicity of BS-RNase to CD34(+) cells was assessed in the direct clonogenic assay using methylcellulose medium of in ex vivo expansion culture supplemented with hematopoietic growth factors. Results. In the clonogenic assay it was shown that BS-RNase completely inhibits growth of both parental NE cells and their sublines resistant to chemotherapeutic drugs at concentrations (up to 50 mu g/ml) which have no significant influence on the growth of colony-forming units, granulocyte macrophage and erythroid burst-forming units. Moreover, BS-RNase had no effect on the ex vivo expansion of total hematopoietic cells or of colony-forming cells from CD34(+) progenitors. Conclusions. BS-RNase is a highly efficient agent against NE cells resistant to chemotherapeutic drugs. The lack of toxicity to hematopoietic progenitor cells suggests that BS-RNase is also likely to have tolerable hematopoietic toxicity. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2001
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