Microarray-based identification of genes and 2-D gel analysis of proteins regulated by Ser/Thr protein kinase of Streptococcus pneumoniae

0100408 - MBU-M 20040097 RIV CZ eng C - Conference Paper (international conference)
Přenosilová, Lenka - Nováková, Linda - Branny, Pavel
Microarray-based identification of genes and 2-D gel analysis of proteins regulated by Ser/Thr protein kinase of Streptococcus pneumoniae.
[Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů.]
European Congress of Clinical Microbiology and Infectious Diseases /14./. Praha, 2004, s. 523.
[European Congress of Clinical Microbiology and Infectious Diseases /14./. Praha (CZ), 01.05.2004-04.05.2004]
Institutional research plan: CEZ:AV0Z5020903
Keywords : pcr * dna
Subject RIV: EE - Microbiology, Virology

To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry.

K identifikaci genů, jejichž exprese je kontrolována funkcí proteinkinasy StkP byla použita metoda DNA čipů. Analýza transkripčních profilů prokázala, že z celkového počtu 2300 genů jich 49 bylo specificky indukováno a 48 reprimováno v mutantu stkP. Výsledky transkriptomové analýzy byly rozšířeny analýzou proteomů a hmotnostní spektrometrii.
Permanent Link: http://hdl.handle.net/11104/0007910