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The initial steps in lincomycin biosynthesis
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SYSNO ASEP 0023838 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title The initial steps in lincomycin biosynthesis Title Úvodní kroky v biosynthése lincomycinu Author(s) Novotná, Jitka (MBU-M)
Honzátko, Aleš (MBU-M)
Bednář, P. (CZ)
Olšovská, Jana (MBU-M)
Kopecký, Jan (MBU-M)
Janata, Jiří (MBU-M) RID, ORCID
Spížek, Jaroslav (MBU-M)Source Title Acta Microbiologica et Immunologica Hungarica. - : Akadémiai Kiadó - ISSN 1217-8950 Pages s. 113-113 Number of pages 1 s. Action Central European Forum for Microbiology /1./ Event date 26.10.2005-28.10.2005 VEvent location Keszthely Country HU - Hungary Event type WRD Language eng - English Country HU - Hungary Keywords lincomycin ; biosynthesis Subject RIV EE - Microbiology, Virology CEZ AV0Z50200510 - MBU-M (2005-2011) Annotation Protein LmbB1, involved in lincomycin biosynthesis in Streptomyces lincolnensis, was expressed in Escherichia coli and purified in its active form. It was found that it is a dimer consisting of identical subunits. By means of capillary electrophoresis and mass spectrometry, chemical structure of the reaction product of this protein was determined. LmbB1 was identified as dioxygenase catalyzing 2,3-extradiol splitting of L-3,4-dihydroxyphenylalanine (DOPA) aromatic ring. Protein LmbB2 was also expressed in E. coli and purified in its active form. It was identified as monooxidase catalyzing hydroxylation of tyrosine to DOPA Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2006
Number of the records: 1