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Rapid protocol for electroporation of Clostridium perfringens
- 1.0021777 - MBÚ 2006 RIV SIGLE NL eng J - Journal Article
Jirásková, Alena - Vítek, L. - Fevery, J. - Ruml, T. - Branny, Pavel
Rapid protocol for electroporation of Clostridium perfringens.
[Rychlý protokol pro elektroporaci Clostridium perfringens.]
Journal of Microbiological Methods. Roč. 62, - (2005), s. 125-127. ISSN 0167-7012. E-ISSN 1872-8359
R&D Projects: GA ČR GA310/02/1436
Institutional research plan: CEZ:AV0Z50200510
Keywords : clostridium perfringens * electroporation * transformation
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.297, year: 2005
A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude
Byl vypracován rychlý a jednoduchý protokol pro elektroporaci Clostridium perfringens plasmidovou DNA. Zavedené modifikace zvyšují účinnost transformace oproti původním hodnotám o jeden řád
Permanent Link: http://hdl.handle.net/11104/0110662
Number of the records: 1