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From the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics

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    SYSNO ASEP0582764
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleFrom the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics
    Author(s) Urbanová, Veronika (BC-A) RID, ORCID
    Lu, S. (US)
    Kalinová, E. (CZ)
    Martins, Larissa Almeida (BC-A) RID, ORCID
    Kozelková, Tereza (BC-A) ORCID
    Dyčka, F. (CZ)
    Ribeiro, J.M. (US)
    Hajdušek, Ondřej (BC-A) RID, ORCID
    Perner, Jan (BC-A) RID, ORCID
    Kopáček, Petr (BC-A) RID, ORCID
    Number of authors10
    Article number104072
    Source TitleInsect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
    Roč. 165, FEB (2024)
    Number of pages15 s.
    Publication formOnline - E
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsmigration inhibitory factor ; ceramide synthase schlank ; horseshoe-crab hemocytes ; gene-expression ; cattle tick ; molecular characterization ; boophilus-microplus ; innate immunity ; hard tick ; dorin-m
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsGA20-05736S GA ČR - Czech Science Foundation (CSF)
    EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingLimited access
    Institutional supportBC-A - RVO:60077344
    UT WOS001154624000001
    EID SCOPUS85182387381
    DOI10.1016/j.ibmb.2024.104072
    AnnotationTicks are blood-feeding arachnids that are known to transmit various pathogenic microorganisms to their hosts. During blood feeding, ticks activate their metabolism and immune system to efficiently utilise nutrients from the host's blood and complete the feeding process. In contrast to insects, in which the fat body is known to be a central organ that controls essential metabolic processes and immune defense mechanisms, the function of the fat body in tick physiology is still relatively unexplored. To fill this gap, we sought to uncover the repertoire of genes expressed in the fat body associated with trachea (FB/Tr) by analyzing the transcriptome of individual, partially fed (previtellogenic) Ixodes ricinus females. The resulting catalog of individual mRNA sequences reveals a broad repertoire of transcripts encoding proteins involved in nutrient storage and distribution, as well as components of the tick immune system. To gain a detailed insight into the secretory products of FB/Tr specifically involved in inter-tissue transport and humoral immunity, the transcriptomic data were complemented with the proteome of soluble proteins in the hemolymph of partially fed female ticks. Among these proteins, the hemolipoglyco-carrier proteins were predominant. When comparing immune peptides and proteins from the fat body with those produced by hemocytes, we found that the fat body serves as a unique producer of certain immune components. Finally, time-resolved transcriptional regulation of selected immune transcripts from the FB/Tr was examined in response to experimental challenges with model microbes and analyzed by RT-qPCR. Overall, our data show that the fat body of ticks, similar to insects, is an important metabolic tissue that also plays a remarkable role in immune defense against invading microbes. These findings improve our understanding of tick biology and its impact on the transmission of tick-borne pathogens.
    WorkplaceBiology Centre (since 2006)
    ContactDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Year of Publishing2025
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S0965174824000031?via%3Dihub
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