Number of the records: 1
From the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics
- 1.
SYSNO ASEP 0582764 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title From the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics Author(s) Urbanová, Veronika (BC-A) RID, ORCID
Lu, S. (US)
Kalinová, E. (CZ)
Martins, Larissa Almeida (BC-A) RID, ORCID
Kozelková, Tereza (BC-A) ORCID
Dyčka, F. (CZ)
Ribeiro, J.M. (US)
Hajdušek, Ondřej (BC-A) RID, ORCID
Perner, Jan (BC-A) RID, ORCID
Kopáček, Petr (BC-A) RID, ORCIDNumber of authors 10 Article number 104072 Source Title Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748
Roč. 165, FEB (2024)Number of pages 15 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords migration inhibitory factor ; ceramide synthase schlank ; horseshoe-crab hemocytes ; gene-expression ; cattle tick ; molecular characterization ; boophilus-microplus ; innate immunity ; hard tick ; dorin-m Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA20-05736S GA ČR - Czech Science Foundation (CSF) EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BC-A - RVO:60077344 UT WOS 001154624000001 EID SCOPUS 85182387381 DOI 10.1016/j.ibmb.2024.104072 Annotation Ticks are blood-feeding arachnids that are known to transmit various pathogenic microorganisms to their hosts. During blood feeding, ticks activate their metabolism and immune system to efficiently utilise nutrients from the host's blood and complete the feeding process. In contrast to insects, in which the fat body is known to be a central organ that controls essential metabolic processes and immune defense mechanisms, the function of the fat body in tick physiology is still relatively unexplored. To fill this gap, we sought to uncover the repertoire of genes expressed in the fat body associated with trachea (FB/Tr) by analyzing the transcriptome of individual, partially fed (previtellogenic) Ixodes ricinus females. The resulting catalog of individual mRNA sequences reveals a broad repertoire of transcripts encoding proteins involved in nutrient storage and distribution, as well as components of the tick immune system. To gain a detailed insight into the secretory products of FB/Tr specifically involved in inter-tissue transport and humoral immunity, the transcriptomic data were complemented with the proteome of soluble proteins in the hemolymph of partially fed female ticks. Among these proteins, the hemolipoglyco-carrier proteins were predominant. When comparing immune peptides and proteins from the fat body with those produced by hemocytes, we found that the fat body serves as a unique producer of certain immune components. Finally, time-resolved transcriptional regulation of selected immune transcripts from the FB/Tr was examined in response to experimental challenges with model microbes and analyzed by RT-qPCR. Overall, our data show that the fat body of ticks, similar to insects, is an important metabolic tissue that also plays a remarkable role in immune defense against invading microbes. These findings improve our understanding of tick biology and its impact on the transmission of tick-borne pathogens. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2025 Electronic address https://www.sciencedirect.com/science/article/pii/S0965174824000031?via%3Dihub
Number of the records: 1