Number of the records: 1  

Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

  1. 1.
    SYSNO ASEP0580758
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleMicrodomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy
    Author(s) Kaňa, Radek (MBU-M) RID, ORCID
    Šedivá, Barbora (MBU-M)
    Prášil, Ondřej (MBU-M) RID, ORCID
    Source TitlePhotosynthetica. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0300-3604
    Roč. 61, Special issue (2023), s. 483-491
    Number of pages9 s.
    Languageeng - English
    CountryCZ - Czech Republic
    KeywordsAiryscan ; cyanobacteria ; frap ; microdomain ; photosystem ; protein mobility ; super-resolution microscopy ; thylakoid membrane heterogeneity
    OECD categoryMicrobiology
    R&D ProjectsGA23-06593S GA ČR - Czech Science Foundation (CSF)
    LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Method of publishingOpen access
    Institutional supportMBU-M - RVO:61388971
    UT WOS001128608100001
    EID SCOPUS85180856825
    DOI10.32615/ps.2023.043
    AnnotationThe investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using superresolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes, countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2024
    Electronic addresshttps://ps.ueb.cas.cz/artkey/phs-202304-0009_microdomains-heterogeneity-in-the-thylakoid-membrane-proteins-visualized-by-super-resolution-microscopy.php
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.