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Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy
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SYSNO ASEP 0580758 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy Author(s) Kaňa, Radek (MBU-M) RID, ORCID
Šedivá, Barbora (MBU-M)
Prášil, Ondřej (MBU-M) RID, ORCIDSource Title Photosynthetica. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0300-3604
Roč. 61, Special issue (2023), s. 483-491Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords Airyscan ; cyanobacteria ; frap ; microdomain ; photosystem ; protein mobility ; super-resolution microscopy ; thylakoid membrane heterogeneity OECD category Microbiology R&D Projects GA23-06593S GA ČR - Czech Science Foundation (CSF) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 001128608100001 EID SCOPUS 85180856825 DOI 10.32615/ps.2023.043 Annotation The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using superresolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes, countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://ps.ueb.cas.cz/artkey/phs-202304-0009_microdomains-heterogeneity-in-the-thylakoid-membrane-proteins-visualized-by-super-resolution-microscopy.php
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