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On the haem auxotrophy of the soft tick Ornithodoros moubata.
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SYSNO ASEP 0580270 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title On the haem auxotrophy of the soft tick Ornithodoros moubata. Author(s) Hatalová, Tereza (BC-A) SAI, ORCID
Erhart, Jan (BC-A)
Kopáček, Petr (BC-A) RID, ORCID
Perner, Jan (BC-A) RID, ORCIDNumber of authors 4 Source Title Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 14, JULY (2023), s. 102170Number of pages 8 s. Publication form Online - E Language eng - English Country DE - Germany Keywords Soft ticks ; Haem biosynthesis ; Ornithodoros Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GM22-18424M GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001169779400001 EID SCOPUS 85150857548 DOI 10.1016/j.ttbdis.2023.102170 Annotation Genomes of ticks display reductions, to various extents, in genetic coding for enzymes of the haem biosynthetic pathway. Here, we mined available transcriptomes of soft tick species and identified transcripts encoding only half of the enzymes involved in haem biosynthesis. Transcripts identified across most species examined were those coding for porphobilinogen synthase, coproporphyrinogen oxidase, protoporphyrinogen oxidase, and ferrochelatase. Genomic retention of porphobilinogen synthase seems to be soft tick-restricted as no such homologue has been identified in any hard tick species. Bioinformatic mining is thus strongly indicative of the lack of biochemical capacity for de novo haem biosynthesis, suggesting a requirement for dietary haem. In the hard tick Ixodes ricinus, depletion of dietary haem, i.e. serum feeding, leads to oviposition of haem-free eggs, with no apparent embryogenesis and larvae formation. In this work, we show that serum-fed Ornithodoros moubata females, unlike those of I. ricinus, laid haem-containing eggs similarly to blood-fed controls, but only by a small proportion of the serum-fed females. To enhance the effect of dietary haem depletion, O. moubata ticks were serum-fed consecutively as last nymphal instars and females. These females laid eggs with profoundly reduced haem deposits, confirming the host origin of the haem. These data confirm the ability of soft ticks to take up and allocate host haem to their eggs in order to drive reproduction of the ticks. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S1877959X23000511?via%3Dihub
Number of the records: 1