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Scalable production of tissue-like vascularized liver organoids from human PSCs
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SYSNO ASEP 0576064 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Scalable production of tissue-like vascularized liver organoids from human PSCs Author(s) Harrison, S.P. (NO)
Siller, R. (NO)
Yoshiaki, T. (US)
Lunov, Oleg (FZU-D) ORCID
Dejneka, Alexandr (FZU-D) RID, ORCIDNumber of authors 36 Source Title Experimental and Molecular Medicine - ISSN 1226-3613
Roč. 55, č. 9 (2023), s. 2005-2024Number of pages 20 s. Language eng - English Country KR - Korea, Republic of Keywords organoids ; drug toxicity ; microscopy ; single-cell RNA sequencing Subject RIV BO - Biophysics OECD category Biophysics Method of publishing Open access Institutional support FZU-D - RVO:68378271 UT WOS 001065142700006 EID SCOPUS 85169166859 DOI 10.1038/s12276-023-01074-1 Annotation The lack of physiological parity between 2D cell culture and in vivo culture has led to the development of more organotypic models, such as organoids. Organoid models have been developed for a number of tissues, including the liver. Current organoid protocols are characterized by a reliance on extracellular matrices (ECMs), patterning in 2D culture, costly growth factors and a lack of cellular diversity, structure, and organization. Current hepatic organoid models are generally simplistic and composed of hepatocytes or cholangiocytes. We have developed an approach that does not require 2D patterning, is ECM independent, and employs small molecules to mimic embryonic liver development that produces large quantities of liver-like organoids. The organoids exhibit key liver functions, including drug metabolism, serum protein production, urea synthesis and coagulation factor production, with preserved post-translational modifications such as N-glycosylation and functionality. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2024 Electronic address https://hdl.handle.net/11104/0346848
Number of the records: 1