Number of the records: 1  

Normalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition

  1. 1.
    SYSNO ASEP0574341
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleNormalized Multipotential Redox Coding of DNA Bases for Determination of Total Nucleotide Composition
    Author(s) Kodr, David (UOCHB-X) ORCID
    Ortiz, M. (ES)
    Sýkorová, Veronika (UOCHB-X) ORCID, RID
    Yenice, C. P. (ES)
    Lesnikowski, Z. J. (PL)
    O’Sullivan, C. K. (ES)
    Hocek, Michal (UOCHB-X) RID, ORCID
    Source TitleAnalytical Chemistry. - : American Chemical Society - ISSN 0003-2700
    Roč. 95, č. 34 (2023), s. 12586-12589
    Number of pages4 s.
    Languageeng - English
    CountryUS - United States
    Keywordsdirect electrochemical detection ; nucleoside triphosphates ; aromatic groups
    OECD categoryOrganic chemistry
    R&D ProjectsGA22-11252S GA ČR - Czech Science Foundation (CSF)
    GX20-00885X GA ČR - Czech Science Foundation (CSF)
    Method of publishingOpen access
    Institutional supportUOCHB-X - RVO:61388963
    UT WOS001050912800001
    EID SCOPUS85169028174
    DOI10.1021/acs.analchem.3c02023
    AnnotationThe previously reported approach of orthogonal multipotential redox coding of all four DNA bases allowed only analysis of the relative nucleotide composition of short DNA stretches. Here, we present two methods for normalization of the electrochemical readout to facilitate the determination of the total nucleotide composition. The first method is based on the presence or absence of an internal standard of 7-deaza-2′-deoxyguanosine in a DNA primer. The exact composition of the DNA was elucidated upon two parallel analyses and the subtraction of the electrochemical signal intensities. The second approach took advantage of a 5′-viologen modified primer, with this fifth orthogonal redox label acting as a reference for signal normalization, thus allowing accurate electrochemical sequence analysis in a single read. Both approaches were tested using various sequences, and the voltammetric signals obtained were normalized using either the internal standard or the reference label and demonstrated to be in perfect agreement with the actual nucleotide composition, highlighting the potential for targeted DNA sequence analysis.
    WorkplaceInstitute of Organic Chemistry and Biochemistry
    Contactasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
    Year of Publishing2024
    Electronic addresshttps://doi.org/10.1021/acs.analchem.3c02023
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.